On Monday and Thursday this week I shadowed sham and shoulder impingement surgeries on young and aged mice. This procedure is used to create a tendinopathy model in the rotator cuff. The RNA sequencing data that I have been examining is from this same experiment, but with smaller cohorts. Four mice per group were used in those earlier experiments and for the procedures I shadowed there were 15 mice per group. It was very interesting getting to see the process of placing the clip in the subacromial space to cause the impingement, and it was very nice getting to talk more with Dr. Eliasberg and the postdoc with whom I am working. On Tuesday, I learned the RNA isolation procedure used by the lab. I will be helping with the processing of the tissue samples over the course of the summer for the shoulder impingement and ACL models. My lab back in Ithaca has done some RNA isolation, so learning this technique will be helpful for my future work. On Friday I will finally get to shadow my first human surgery with Dr. Eliasberg. Next week, I will be shadowing procedures on mice using ultrasound to look at tendon properties. I am excited for this as some of my previous research used focus ultrasound for therapeutic delivery, so I am interested in seeing other ways it can be used in research. Additionally, I also finally received Dr. Rodeo's schedule and will be shadowing him in the clinic and in surgery. In July, I have also schedule a couple of days where I will get to shadow Dr. Eliasberg in the clinic. I am very much looking forward to getting to do more on the clinical side of this program.
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