This week, I attended multiple seminars: Role of Subchondral Bone in Osteoarthritis Development (Dr. Marjolein van der Meulen), Salt Inducible Kinase (SIK) inhibition in mice with long bone fractures (Dr. Mark Bubnovich), and Metabolic Regulation of Muscle Regeneration (Dr. Shihuan Kuang). Additionally, I made some progress on my research—I finally received sectioned samples of human patients from ACL reconstruction, total knee arthroplasty, or total knee arthroplasty revision, which I will be using to study the correlation between patient stiffness, fibrosis, and cellular senescence. I was able to stain the samples with H&E, and will be using a slide scanner (which I got trained on this week!) to image all of the slides next week. Further, I confirmed that my IHC protocol for staining uPAR (a marker of senescence) works on mouse knees, meaning that, once I order and receive an antibody for human uPAR (since there isn’t much cross-reaction between human and mouse uPAR), I will be able to stain the human patient samples. Lastly, I observed an arthroscopic hip surgery with Dr. Stephanie Buza—I am definitely a bigger fan of arthroscopic surgeries compared to open surgeries, since both the blood and smell are a little overwhelming with open surgeries, so this was an awesome experience to see another surgeon operate.
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This week was heavy in the clinic and OR. We started out with attending the weekly multidisciplinary tumor board on Monday. Doctors discusse...
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Compared to last week, this week was more research-oriented than clinic-focused. When meeting with patients this week, I saw a couple intere...
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This week I with Christina, one of the lab techs between Dr. Carli and Dr. Bostrom. We prepared screws that have biofilm on them for SEM at ...
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