Week 6: Allison

I spent the first part of this week running flow cytometry on the T cell samples I stained last week and started learning FlowJo to perform the analysis. Eleana and I also worked on an experimental plan for our next set of experiments and discussed and refined it at lab meeting. We also worked on preparing more gels for T cell activation, and earlier today we extracted and seeded T cells from the spleens and lymph nodes of mice into our gels so they activate over the weekend. We figured out how to make our own coculturing inserts for well plates so T cells and cancer cells could be seeded in different chambers and imaged as they migrate toward each other. We were hoping to buy inserts that would suit these needs, but the shipping would take over a month, so we made our own out of PDMS and Legos. I had another day of shadowing in pathology this week, and I'm always surprised how different cases look even for the same types of breast cancer.