We received our patellar tendon samples back from the Histopathology Core on Monday, which Hamilton and I imaged before progressing to an H&E stain. After scanning the slides post H&E, Hamilton and I familiarized ourselves with the tendon extracellular matrix grading system (The Movin Score) developed by Wu et al. in 2011. The grading scale goes through many different criteria for a comprehensive analysis of tendon health. The collagen fibers that compose tendon are evaluated based on structure and alignment, while local tenocyte properties such as cellularity and circularity are also taken into account.
The majority of our work this week and moving forward will be recapturing some higher focus images at the proximal, midsubstance, and distal regions of the patellar tendon. Each image will be assigned a Movin Score, which can be compared across timepoints to determine the long-term effects of collagenase-induced tendinopathy.
Wu et al. 2011: https://link.springer.com/article/10.1007/s00402-010-1157-5
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