For the final week of immersion, I was able to start working with some of the multiplexed immunofluorescence data from the run we did last week. It seems that some of the antibodies were not quite at the correct dilution, so we are making some changes to the dilution factors for the panel and we will run the experiment again soon. I am glad that this immersion project will open doors to some new collaborations which will continue after I leave the city. I spent a lot of time on the microtome this week, sectioning more tissue samples for future experiments, which should save me some time down the road. It was also good to get additional practice on the microtome before I leave. I spent all day Wednesday shadowing in the OR, and saw several different procedures, including a brow lift, a skin graft, a mammectomy, and a panniculectomy. The variety of procedures that they see on the plastic surgery service is quite interesting. From some of the procedures we were able to collect some more tissue, and I helped to process this tissue for the biobank.
Friday, July 25, 2025
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Week 8: Eleana
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