The HSS Pathology Core sectioned some of the collagenase-induced patellar tendinopathy samples due to our broken microtome. Hamilton and I have completed a Hemotoxin and Eosin (H&E) stain to differentiate the tissue types present in the sample alongside staining cell nuclei. We expect to see a decrease in cells present within the tendon over longer courses of collagenase treatment due to increased extracellular matrix degradation.
We've noticed that the Pathology Core may of not cut deeply enough into the sample, which is apparent since the patella is not present when observed under microscope. While the samples were not at the appropriate depth, we already began visualizing collagen degradation along the edges of the patellar tendon. Once the microtome is repaired, we can begin generating more samples deeper into the tissue. I worked through some physics calculations for the drop tower-induced tendinopathy model and completed my code for an automated height adjuster. I'm working to generate a functional circuit through Tinkercad for some strain gauges and force sensors for this project as well.
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